本书旨在描述各种技术，反映了目前冠状病毒学领域的广泛研究，首先概述了目前对冠状病毒复制和发病机制的理解，向专家和非专家介绍该领域。本书的其余部分分为几个章节，首先是描述冠状病毒的鉴定、诊断和研究冠状病毒的进化的章节。接下来的几章讨论了对冠状病毒繁殖有用的细胞和器官培养物的制备和滴定技术，以及需要纯化病毒的病毒功能分析技术。接下来的章节介绍了两种常用的冠状病毒反向遗传学技术，以及详细介绍了细胞受体的鉴定、病毒附着蛋白的结合谱以及病毒与细胞融合的技术。最后几章涵盖了鉴定病毒-宿主蛋白-蛋白相互作用的广泛技术，确认这些蛋白在病毒复制中的功能作用，通过全基因组或途径特异性方法研究宿主细胞反应，以及可视化病毒复制复合物。本书采用非常成功的《分子生物学方法》系列格式编写，各章包含了详细的描述和实施建议，这对于在实验室中获得最佳结果至关重要。
权威而实用的《冠状病毒。方法和协议》吸引了广大科学家，因为它突出了目前在冠状病毒学领域使用的技术，同时也讨论了适用于其他病毒学领域的实践。
This volume aims to describe a variety of techniques that reflects the wide range of research currently performed in the field of coronavirology, and begins with an overview of current understandings of coronavirus replication and pathogenesis to introduce specialists and non-specialists to the field. The rest of the book is divided into several sections of chapters beginning with those that describe identification, diagnosis, and study of the evolution of coronaviruses. The next few chapters discuss the preparation of cells and organ cultures useful in propagating coronaviruses and titration techniques, as well as techniques for analyzing virus functions that require purification of the viruses. The next chapters describe two commonly used reverse genetics techniques for coronaviruses, and techniques detailing identification of cellular receptors, binding profiles of viral attachment proteins, and virus-cell fusion. The final chapters cover a broad spectrum of techniques to identify virus-host protein-protein interactions, confirm the functional role of these proteins in virus replication, study host cell responses through genome-wide or pathway-specific approaches, and visualize virus replication complexes. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.
Authoritative and practical, Coronaviruses: Methods and Protocols appeals to a wide variety of scientists because it highlights techniques that are currently used in the coronovirology field, while also discussing practices applicable to other virology fields.
